DNA Cloning Service

Binaries

p7o-LH-UbiAt-ocs C335

Ligation of 204pUbiAtmu-ocs* into B414p7o-LH*

12345678910pVS1Pro-Ubq10AtSm/SpColE135SBarIntE9bomBarOCSSTLS1RBLBC335p7o-LH-UbiAt10,861 bp
Promoter 1
35s
Gene 1
bar
Promoter 2
Ubq10
Gene 2
MCS
Features
dicots
Size
10,861 bp, circular
Record date
29-SEP-2017

Files

Order

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
LB T-DNA border misc_feature 12424 +
35S Promoter misc_feature 309724416 +
Bar Selection marker CDS 745978234 +
STLS1 Other misc_feature 9791,167189 +
Bar Selection marker CDS 1,1681,483316 +
E9 Terminator misc_feature 1,4961,786291 +
Pro-Ubq10At Promoter misc_feature 1,8353,3101,476 +
Int Site misc_feature 3,0053,308304 +
OCS Terminator misc_feature 3,3583,553196 +
RB T-DNA border misc_feature 3,6383,66124 +
ColE1 Origin / backbone misc_feature 4,1964,893698 +
bom Origin / backbone misc_feature 4,9305,189260 +
pVS1 Origin / backbone misc_feature 5,4429,1473,706 +
Sm/Sp Selection marker CDS 9,33110,3411,011 +

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.