DNA Cloning Service

Binaries

p7oU10-35s-ocs-LH B467

Ligation of p35-OCS-BM* into B434p7oU10-LH*

12345678910pVS1UBQ10Sm/SpColE1P35SbarintTE9bombarTocsintUbi cds 5RBLBB467p7oU10-35s-o10,872 bp
Promoter 1
Ubq10
Gene 1
bar
Promoter 2
35s
Gene 2
MCS
Features
dicots
Size
10,872 bp, circular
Record date
08-FEB-2016

Files

Order

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
LB T-DNA border misc_feature 12424 +
UBQ10 Other misc_feature 3091,7841,476 +
Ubi cds 5 Promoter misc_feature 1,4131,47664 +
int Site misc_feature 1,4791,782304 +
bar Selection marker CDS 1,8042,037234 +
int Site misc_feature 2,0382,226189 +
bar Selection marker CDS 2,2272,542316 +
TE9 Terminator misc_feature 2,5552,845291 +
P35S Promoter misc_feature 2,9003,322423 +
Tocs Terminator misc_feature 3,3693,564196 +
RB T-DNA border misc_feature 3,6493,67224 +
ColE1 Origin / backbone misc_feature 4,2074,904698 +
bom Origin / backbone misc_feature 4,9415,200260 +
pVS1 Origin / backbone misc_feature 5,4539,1583,706 +
Sm/Sp Selection marker CDS 9,34210,3521,011 +

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.