DNA Cloning Service

Binaries

p7oU10-oGFPint B437

Ligation of B444pmGFPD-ocs* into B434p7oU10-LH*

1234567891011pVS1UBQ10Sm/SpColE1gfpgfpbarintTE9bombarTocsintintUbi cds 5RBLBB437p7oU10-oGFPi11,364 bp
Promoter 1
Ubq10
Gene 1
bar
Gene 2
link-EGFP
Features
dicots
Size
11,364 bp, circular
Record date
22-JAN-2016

Files

Order

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
LB T-DNA border misc_feature 12424 +
UBQ10 Other misc_feature 3091,7841,476 +
Ubi cds 5 Promoter misc_feature 1,4131,47664 +
int Site misc_feature 1,4791,782304 +
bar Selection marker CDS 1,8042,037234 +
int Site misc_feature 2,0382,226189 +
bar Selection marker CDS 2,2272,542316 +
TE9 Terminator misc_feature 2,5552,845291 +
gfp Coding sequence CDS 2,9473,325379 +
int Site CDS 3,3263,514189 +
gfp Coding sequence CDS 3,5153,852338 +
Tocs Terminator misc_feature 3,8614,056196 +
RB T-DNA border misc_feature 4,1414,16424 +
ColE1 Origin / backbone misc_feature 4,6995,396698 +
bom Origin / backbone misc_feature 5,4335,692260 +
pVS1 Origin / backbone misc_feature 5,9459,6503,706 +
Sm/Sp Selection marker CDS 9,83410,8441,011 +

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.