DNA Cloning Service

Binaries

p935sGFP B982

Ligation of NptGFP-Xho-Mlu* into B414p7o-LH*

12345678910pVS1Sm/SpColE1NptIIo35SGFPmGFPmNptIIoE9bomIntIntRBLBB982p935sGFP10,390 bp
Promoter 1
35s
Gene 1
nptII-EGFP
Features
dicots
Size
10,390 bp, circular
Record date
04-OCT-2017

Files

Order

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
LB T-DNA border misc_feature 12424 +
35S Promoter misc_feature 309724416 +
NptIIo Selection marker CDS 7451,043299 +
Int Site CDS 1,0441,230187 +
NptIIo Selection marker CDS 1,2311,734504 +
GFPm Coding sequence CDS 1,7862,164379 +
Int Site CDS 2,1652,353189 +
GFPm Coding sequence CDS 2,3542,691338 +
E9 Terminator misc_feature 2,7012,991291 +
RB T-DNA border misc_feature 3,1673,19024 +
ColE1 Origin / backbone misc_feature 3,7254,422698 +
bom Origin / backbone misc_feature 4,4594,718260 +
pVS1 Origin / backbone misc_feature 4,9718,6763,706 +
Sm/Sp Selection marker CDS 8,8609,8701,011 +

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.