DNA Cloning Service

Binaries

p9UbiGfp B983

Ligation of NptGFP-Xho-Mlu* into B434p7oU10-LH*

1234567891011pVS1Pro-UbiSm/SpColE1NptIIoGFPmGFPmIntNptIIoE9bomIntIntRBLBB983p9UbiGfp11,449 bp
Promoter 1
Ubq10
Gene 1
nptII-EGFP
Features
dicots
Size
11,449 bp, circular
Record date
04-OCT-2017

Files

Order

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
LB T-DNA border misc_feature 12424 +
Pro-Ubi Promoter misc_feature 3091,7841,476 +
Int Site misc_feature 1,4791,782304 +
NptIIo Selection marker CDS 1,8042,102299 +
Int Site CDS 2,1032,289187 +
NptIIo Selection marker CDS 2,2902,793504 +
GFPm Coding sequence CDS 2,8453,223379 +
Int Site CDS 3,2243,412189 +
GFPm Coding sequence CDS 3,4133,750338 +
E9 Terminator misc_feature 3,7604,050291 +
RB T-DNA border misc_feature 4,2264,24924 +
ColE1 Origin / backbone misc_feature 4,7845,481698 +
bom Origin / backbone misc_feature 5,5185,777260 +
pVS1 Origin / backbone misc_feature 6,0309,7353,706 +
Sm/Sp Selection marker CDS 9,91910,9291,011 +

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.