DNA Cloning Service

Binaries

p9o-LH-35s-ocs B425

Ligation of p35-OCS-BM* into B415p9o-LH*

12345678910pVS1Sm/SpColE1nptIIoP35SP35SnptIIoTE9bomTocsintRBLBB425p9o-LH-35s-o10,065 bp
Promoter 1
35s
Gene 1
nptII
Promoter 2
35s
Gene 2
MCS
Features
dicots
Size
10,065 bp, circular
Record date
22-JAN-2016

Files

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Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
LB T-DNA border misc_feature 12424 +
P35S Promoter misc_feature 309724416 +
nptIIo Selection marker CDS 7451,043299 +
splice Site misc_signal 1,0401,0434 +
int Site CDS 1,0441,230187 +
splice Site misc_signal 1,2291,2324 +
nptIIo Selection marker CDS 1,2311,734504 +
TE9 Terminator misc_feature 1,7482,038291 +
P35S Promoter misc_feature 2,0932,515423 +
Tocs Terminator misc_feature 2,5622,757196 +
RB T-DNA border misc_feature 2,8422,86524 +
ColE1 Origin / backbone misc_feature 3,4004,097698 +
bom Origin / backbone misc_feature 4,1344,393260 +
pVS1 Origin / backbone misc_feature 4,6468,3513,706 +
Sm/Sp Selection marker CDS 8,5359,5451,011 +

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.