DNA Cloning Service

Binaries

p9o2x35s-UbiZm-LH B516

Ligation of 594pUbi-BA* into B485p9o2x35s-LH*

1234567891011pVS1UbiZmSm/SpP2x35sColE1intnptIIonptIIoTE9TnosintRBLBB516p9o2x35s-Ubi11,647 bp
Promoter 1
2x35s
Gene 1
nptII
Promoter 2
UbiZm
Gene 2
MCS
Features
monocot
Size
11,647 bp, circular
Record date
22-JAN-2016

Files

Order

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
LB T-DNA border misc_feature 12424 +
P2x35s Promoter misc_feature 3141,098785 +
nptIIo Selection marker CDS 1,1161,414299 +
int Site CDS 1,4151,601187 +
nptIIo Selection marker CDS 1,6022,108507 +
TE9 Terminator misc_feature 2,1262,416291 +
UbiZm Promoter misc_feature 2,4803,9871,508 +
int Site misc_feature 3,4663,985520 +
Tnos Terminator misc_feature 4,0484,301254 +
RB T-DNA border misc_feature 4,4244,44724 +
ColE1 Origin / backbone misc_feature 4,9825,679698 +
pVS1 Origin / backbone misc_feature 6,2289,9333,706 +
Sm/Sp Selection marker CDS 10,11711,1271,011 +

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.