DNA Cloning Service

Binaries

p9oU10-35s-ocs-LH B468

Ligation of p35-OCS-BM* into B435p9oU10-LH*

1234567891011pVS1UBQ10Sm/SpColE1nptIIoP35SintnptIIoTE9bomTocsintUbi cds 5RBLBB468p9oU10-35s-o11,124 bp
Promoter 1
Ubq10
Gene 1
nptII
Promoter 2
35s
Gene 2
MCS
Features
dicots
Size
11,124 bp, circular
Record date
22-JAN-2016

Files

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Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
LB T-DNA border misc_feature 12424 +
UBQ10 Other misc_feature 3091,7841,476 +
Ubi cds 5 Promoter misc_feature 1,4131,47664 +
int Site misc_feature 1,4791,782304 +
nptIIo Selection marker CDS 1,8042,102299 +
splice Site misc_signal 2,0992,1024 +
int Site CDS 2,1032,289187 +
splice Site misc_signal 2,2882,2914 +
nptIIo Selection marker CDS 2,2902,793504 +
TE9 Terminator misc_feature 2,8073,097291 +
P35S Promoter misc_feature 3,1523,574423 +
Tocs Terminator misc_feature 3,6213,816196 +
RB T-DNA border misc_feature 3,9013,92424 +
ColE1 Origin / backbone misc_feature 4,4595,156698 +
bom Origin / backbone misc_feature 5,1935,452260 +
pVS1 Origin / backbone misc_feature 5,7059,4103,706 +
Sm/Sp Selection marker CDS 9,59410,6041,011 +

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.