p9oU10-oGFPint B438

Ligation of B444pmGFPD-ocs* into B435p9oU10-LH*

Files

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Feature	Type	Start	End	Length	Strand
LB	T-DNA border misc_feature	1	24	24	+
UBQ10	Other misc_feature	309	1,784	1,476	+
Ubi cds 5	Promoter misc_feature	1,413	1,476	64	+
int	Site misc_feature	1,479	1,782	304	+
nptIIo	Selection marker CDS	1,804	2,102	299	+
splice	Site misc_signal	2,099	2,102	4	+
int	Site CDS	2,103	2,289	187	+
splice	Site misc_signal	2,288	2,291	4	+
nptIIo	Selection marker CDS	2,290	2,793	504	+
TE9	Terminator misc_feature	2,807	3,097	291	+
gfp	Coding sequence CDS	3,199	3,577	379	+
int	Site CDS	3,578	3,766	189	+
gfp	Coding sequence CDS	3,767	4,104	338	+
Tocs	Terminator misc_feature	4,113	4,308	196	+
RB	T-DNA border misc_feature	4,393	4,416	24	+
ColE1	Origin / backbone misc_feature	4,951	5,648	698	+
bom	Origin / backbone misc_feature	5,685	5,944	260	+
pVS1	Origin / backbone misc_feature	6,197	9,902	3,706	+
Sm/Sp	Selection marker CDS	10,086	11,096	1,011	+

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.