DNA Cloning Service

Binaries

p9oUZm-LH B480

Ligation of UbiZm-Bsa* into B475p9oM-LH*

12345678910pVS1UbiZmSm/SpColE1intnptIIonptIIoTE9intRBLBB480p9oUZm-LH10,537 bp
Promoter 1
UbiZm
Gene 1
nptII
Features
monocot
Size
10,537 bp, circular
Record date
26-JAN-2016

Files

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Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
UbiZm Coding sequence CDS 621,5691,508 +
int Site misc_feature 1,0491,568520 +
nptIIo Selection marker CDS 1,5901,888299 +
int Site CDS 1,8892,075187 +
nptIIo Selection marker CDS 2,0762,582507 +
TE9 Terminator misc_feature 2,6002,890291 +
RB T-DNA border misc_feature 3,0663,08924 +
ColE1 Origin / backbone misc_feature 3,6244,321698 +
pVS1 Origin / backbone misc_feature 4,8708,5753,706 +
Sm/Sp Selection marker CDS 8,7599,7691,011 +
LB T-DNA border misc_feature 10,29010,31324 +

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.