DNA Cloning Service

Binaries

pRedU10-35s-ocs-LH B469

Ligation of Red-E9-Xho-Pst* into B468p9oU10-35s-ocs-LH*

12345678910pVS1UBQ10Sm/SpColE1dsRedP35SintTE9bomTocsUbi cds 5RBLBB469pRedU10-35s-10,823 bp
Promoter 1
Ubq10
Gene 1
dsRed
Promoter 2
35s
Gene 2
MCS
Features
dicots
Size
10,823 bp, circular
Record date
22-JAN-2016

Files

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Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
LB T-DNA border misc_feature 12424 +
UBQ10 Other misc_feature 3091,7841,476 +
Ubi cds 5 Promoter misc_feature 1,4131,47664 +
int Site misc_feature 1,4791,782304 +
dsRed Coding sequence CDS 1,8042,499696 +
change to T Site misc_signal 2,1602,1601 +
TE9 Terminator misc_feature 2,5162,796281 +
P35S Promoter misc_feature 2,8513,273423 +
Tocs Terminator misc_feature 3,3203,515196 +
RB T-DNA border misc_feature 3,6003,62324 +
ColE1 Origin / backbone misc_feature 4,1584,855698 +
bom Origin / backbone misc_feature 4,8925,151260 +
pVS1 Origin / backbone misc_feature 5,4049,1093,706 +
Sm/Sp Selection marker CDS 9,29310,3031,011 +

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.