p7D35s-C9wt-U6At D023

Ligation of C035p7ioR-Uq10CasWToi* into D011p7D35s*

Files

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Feature	Type	Start	End	Length	Strand
LB'	T-DNA border misc_feature	1	24	24	+
P35S	Promoter misc_feature	169	584	416	+
Bar	Selection marker CDS	605	838	234	+
STLS1	Other misc_feature	839	1,027	189	+
Bar	Selection marker CDS	1,028	1,343	316	+
TE9	Terminator misc_feature	1,356	1,646	291	+
Pro-Ubq10At	Promoter misc_feature	1,672	3,147	1,476	+
Ubi cds 5	Promoter misc_feature	2,776	2,839	64	+
íntron	Other misc_feature	2,842	3,145	304	+
'Cas9WT	Coding sequence CDS	3,169	7,365	4,197	+
NLS	Site misc_feature	3,175	3,195	21	+
NLS	Site misc_feature	7,315	7,362	48	+
NOS-T	Terminator misc_feature	7,371	7,624	254	+
U6At	Promoter misc_feature	7,634	8,078	445	+
targetRNA	Site CDS	8,079	8,105	27	+
hairpin	Site CDS	8,106	8,135	30	+
gRNA	Site misc_feature	8,106	8,188	83	+
Right Border Region	Other misc_feature	8,234	8,836	603	+
RB	T-DNA border misc_feature	8,279	8,302	24	+
ColE1	Origin / backbone misc_feature	8,850	9,508	659	+
''pVS1 ORI'''	Origin / backbone misc_feature	9,537	11,500	1,964	+
ParB	Coding sequence CDS	9,863	11,038	1,176	+
strep/spec	Selection marker CDS	11,689	12,699	1,011	+
Left Border Region	T-DNA border misc_feature	12,767	163	0	+

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.