p7ioRAct-UZmCwtoi C232

Ligation of B489p7oAct-LH* into C143p6ioRAct-UZmCwtoi*

Files

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Feature	Type	Start	End	Length	Strand
LB	T-DNA border misc_feature	1	24	24	+
pAct'	Promoter misc_feature	313	1,710	1,398	+
Bar'	Selection marker CDS	1,738	1,971	234	+
STLS1	Other misc_feature	1,972	2,160	189	+
''Bar'	Selection marker CDS	2,161	2,478	318	+
'E9	Terminator misc_feature	2,498	2,788	291	+
Ubi-int	Promoter misc_feature	2,843	4,351	1,509	+
Intron	Site misc_feature	3,830	4,349	520	+
NLS	Site misc_feature	4,382	4,402	21	+
'Cas9WT	Coding sequence CDS	4,418	8,572	4,155	+
NLS	Site misc_feature	8,522	8,569	48	+
NOS-T	Terminator misc_feature	8,578	8,831	254	+
U6Os'	Promoter misc_feature	8,841	9,293	453	+
insert target RNA	Site CDS	9,294	9,322	29	+
hairpin	Site CDS	9,323	9,352	30	+
gRNA scaffold	Site misc_feature	9,323	9,405	83	+
RB	T-DNA border misc_feature	9,491	9,514	24	+
ColE1	Origin / backbone misc_feature	10,049	10,746	698	+
'pVS1	Origin / backbone misc_feature	11,034	13,821	2,788	+
Sm/Sp	Selection marker CDS	14,005	15,015	1,011	+

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.