p9ioR-35sCasWToi B764
Ligation of Cas9WToi Bam, Nru* into B357p9ioR-35sCasWT*
- Promoter 1
- 35s
- Gene 1
- nptII
- Promoter 2
- 35s
- Gene 2
- Cas9
- Features
- U6At
- Size
- 13,681 bp, circular
- Record date
- 11-SEP-2017
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| LB | T-DNA border misc_feature | 1 | 24 | 24 | + |
| 35S | Promoter misc_feature | 309 | 724 | 416 | + |
| NptII | Site CDS | 741 | 777 | 37 | + |
| int | Site misc_feature | 778 | 966 | 189 | + |
| NptII | Selection marker CDS | 967 | 1,733 | 767 | + |
| 'E9 | Terminator misc_feature | 1,744 | 2,034 | 291 | + |
| 35S-Pro | Promoter misc_feature | 2,089 | 2,506 | 418 | + |
| TATA-box | Site CDS | 2,477 | 2,483 | 7 | + |
| NLS | Site misc_feature | 2,538 | 2,558 | 21 | + |
| 'Cas9WT | Coding sequence CDS | 2,538 | 6,728 | 4,191 | + |
| int' | Site misc_feature | 2,949 | 2,956 | 8 | + |
| NLS | Site misc_feature | 6,678 | 6,725 | 48 | + |
| NOS-T | Terminator misc_feature | 6,734 | 6,987 | 254 | + |
| 'RNA' | Other misc_feature | 6,992 | 7,552 | 561 | + |
| U6At | Coding sequence CDS | 6,997 | 7,441 | 445 | + |
| targetRNA | Site CDS | 7,442 | 7,468 | 27 | + |
| hairpin | Site CDS | 7,469 | 7,498 | 30 | + |
| gRNA | Site misc_feature | 7,469 | 7,551 | 83 | + |
| RB | T-DNA border misc_feature | 7,637 | 7,660 | 24 | + |
| ColE1 | Origin / backbone misc_feature | 8,195 | 8,892 | 698 | + |
| 'pVS1 | Origin / backbone misc_feature | 9,180 | 11,967 | 2,788 | + |
| Sm/Sp | Selection marker CDS | 12,151 | 13,161 | 1,011 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.