p9ioR-U10CasD10A D029
Ligation of B763p9ioR-35sD10Aoi* into C111p9ioR-Uq10CasWToi*
- Promoter 1
- 35s
- Gene 1
- nptII
- Promoter 2
- Ubq10
- Gene 2
- Cas9 nick
- Features
- Dicots
- Size
- 14,739 bp, circular
- Record date
- 26-AUG-2020
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| LB | T-DNA border misc_feature | 1 | 24 | 24 | + |
| P35S | Promoter misc_feature | 309 | 724 | 416 | + |
| NptIIo | Selection marker CDS | 745 | 1,043 | 299 | + |
| int | Site CDS | 1,044 | 1,230 | 187 | + |
| NptIIo | Selection marker CDS | 1,231 | 1,734 | 504 | + |
| TE9 | Terminator misc_feature | 1,748 | 2,038 | 291 | + |
| Pro-Ubq10At | Promoter misc_feature | 2,087 | 3,562 | 1,476 | + |
| Ubi cds 5 | Promoter misc_feature | 3,191 | 3,254 | 64 | + |
| íntron | Other misc_feature | 3,257 | 3,560 | 304 | + |
| D10A' | Coding sequence CDS | 3,584 | 7,786 | 4,203 | + |
| NLS | Site misc_feature | 3,590 | 3,610 | 21 | + |
| D10A mut to C | Site misc_feature | 3,660 | 3,660 | 1 | + |
| NLS | Site misc_feature | 7,736 | 7,783 | 48 | + |
| NOS-T | Terminator misc_feature | 7,792 | 8,045 | 254 | + |
| U6At | Promoter misc_feature | 8,055 | 8,499 | 445 | + |
| targetRNA | Site CDS | 8,500 | 8,526 | 27 | + |
| hairpin | Site CDS | 8,527 | 8,556 | 30 | + |
| gRNA | Site misc_feature | 8,527 | 8,609 | 83 | + |
| RB | T-DNA border misc_feature | 8,695 | 8,718 | 24 | + |
| ColE1 | Origin / backbone misc_feature | 9,253 | 9,950 | 698 | + |
| pVS1 | Origin / backbone misc_feature | 10,238 | 13,025 | 2,788 | + |
| Sm/Sp | Selection marker CDS | 13,209 | 14,219 | 1,011 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.