p35s-mGFPD-ocs B589
Ligation of B444pmGFPD-ocs* into B491p35s-DoGusi-ocs*
- Promoter 2
- 35s
- Gene 2
- GFP
- Features
- dicots
- Size
- 4,489 bp, circular
- Record date
- 22-JAN-2016
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| P35S | Promoter misc_feature | 144 | 562 | 419 | + |
| Minimal promotor | Site misc_signal | 473 | 562 | 90 | + |
| T-start | Site misc_feature | 563 | 564 | 2 | + |
| gfp | Coding sequence CDS | 590 | 968 | 379 | + |
| int | Site TATA_signal | 969 | 1,157 | 189 | + |
| gfp | Coding sequence CDS | 1,158 | 1,495 | 338 | + |
| Tocs | Terminator misc_feature | 1,504 | 1,699 | 196 | + |
| Amp | Selection marker misc_feature | 2,515 | 3,375 | 861 | + |
| ColE1 | Origin / backbone CDS | 3,418 | 4,134 | 717 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.