DNA Cloning Service

pUC

pActOs-LGFP B635

Ligation of B629p35s-LGFP* into B627pAct-ocs*

12345ACTAmpColE1mRNAintgfpmgfpmOCSintlinkB635pActOs-LGFP5,526 bp
Promoter 2
ActOs
Gene 2
Link-GFP
Features
monocots
Size
5,526 bp, circular
Record date
08-FEB-2016

Files

Order

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
ACT Promoter misc_feature 1031,5001,398 +
mRNA Coding sequence CDS 9461,500555 +
int Site misc_feature 1,0361,497462 +
link Other misc_feature 1,5491,59951 +
gfpm Coding sequence CDS 1,6001,978379 +
int Site CDS 1,9792,167189 +
gfpm Coding sequence CDS 2,1682,505338 +
OCS Terminator misc_feature 2,5152,710196 +
Amp Selection marker misc_feature 3,5264,386861 +
ColE1 Origin / backbone misc_feature 4,4295,145717 +

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.