DNA Cloning Service

pUC

pUbiAt-mGFPD-ocs B592

Ligation of B494pUbiAt-DoGusi-ocs* into B444pmGFPD-ocs*

12345UBQ10AmpColE1gfpgfpintTocsintUbi cds 5B592pUbiAt-mGFPD5,544 bp
Promoter 2
Ubq10
Gene 2
GFP
Features
dicots
Size
5,544 bp, circular
Record date
22-JAN-2016

Files

Order

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
UBQ10 Other misc_feature 461,5211,476 +
Ubi cds 5 Promoter misc_feature 1,1501,21364 +
int Site misc_feature 1,2161,519304 +
gfp Coding sequence CDS 1,5471,925379 +
int Site CDS 1,9262,114189 +
gfp Coding sequence CDS 2,1152,452338 +
Tocs Terminator misc_feature 2,4612,656196 +
Amp Selection marker misc_feature 3,4724,332861 +
ColE1 Origin / backbone CDS 4,3755,091717 +

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.