pUbiHa-mGFPD-ocs B591

Ligation of B444pmGFPD-ocs* into B493pUbiHa-DoGusi-ocs*

Files

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Feature	Type	Start	End	Length	Strand
UbiHa	Promoter misc_feature	146	481	336	+
cat	Site misc_signal	263	265	3	+
cat	Site misc_signal	266	268	3	+
cat	Site misc_signal	299	301	3	+
cat	Site misc_signal	328	330	3	+
cat	Site misc_signal	339	341	3	+
cat	Site misc_signal	360	362	3	+
cat	Site misc_signal	363	365	3	+
tata	Site misc_signal	368	371	4	+
mRNA	Coding sequence CDS	399	481	83	+
gfp	Coding sequence CDS	507	885	379	+
int	Site CDS	886	1,074	189	+
gfp	Coding sequence CDS	1,075	1,412	338	+
Tocs	Terminator misc_feature	1,421	1,616	196	+
Amp	Selection marker misc_feature	2,432	3,292	861	+
ColE1	Origin / backbone CDS	3,335	4,051	717	+

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.