DNA Cloning Service

pUC

pUbiZm-LmEGFP D390

Ligation of GFP-Hind-Sal* into B633pUbiZm-LGFP*

12345PUbiAmpColE1IntronmEGFPmEGFPOCSST-LS1linkD390pUbiZm-LmEGF5,641 bp
Promoter 1
Ubi
Gene 1
mEGFP
Features
monocots
Size
5,641 bp, circular
Record date
26-AUG-2020

Files

Order

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
PUbi Promoter misc_feature 1061,6141,509 +
Intron Site misc_feature 1,0931,612520 +
link Site misc_feature 1,6641,71451 +
mEGFP Coding sequence CDS 1,7152,093379 +
ST-LS1 Coding sequence CDS 2,0942,282189 +
mEGFP Coding sequence CDS 2,2832,620338 +
A206K Site misc_feature 2,5192,5213 +
OCS Terminator misc_feature 2,6302,825196 +
Amp Selection marker misc_feature 3,6414,501861 +
ColE1 Origin / backbone CDS 4,5445,260717 +

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.