DNA Cloning Service

pUC

pUbiZm-YFPiM B728

Ligation of PCR1* into B595pUbiZm-mGFPM-ocs*

12345pUbiAmpColE1IntYFP''YFPOCSIntB728pUbiZm-YFPiM5,583 bp
Promoter 2
UbiZm
Gene 2
mCitrine
Features
monocots
Size
5,583 bp, circular
Record date
04-OCT-2017

Files

Order

Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.

Annotated features

FeatureTypeStartEndLengthStrand
pUbi Promoter misc_feature 1221,6291,508 +
Int Site misc_feature 1,1081,627520 +
YFP' Coding sequence CDS 1,6552,036382 +
Int Site CDS 2,0372,225189 +
'YFP Coding sequence CDS 2,2262,563338 +
OCS Terminator misc_feature 2,5722,767196 +
Amp Selection marker misc_feature 3,5834,443861 +
ColE1 Origin / backbone CDS 4,4865,202717 +

PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite

Feature types are inferred from the labels in the GenBank file; the file itself is the reference.