p75-GusRNAi
Ligation of 35GusRNAi into pLH7500
- Promoter 1
- 35S
- Gene 1
- bar
- Promoter 2
- 35S
- Gene 2
- Gus-spacer-Gus
- Features
- hairpin construct with spacer, for RNAi
- Size
- 11,808 bp, circular
- Record date
- 07-JAN-2002
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| 35S | Promoter misc_feature | 7 | 422 | 416 | + |
| pat | Selection marker misc_feature | 439 | 990 | 552 | + |
| T35S | Terminator misc_feature | 1,013 | 1,213 | 201 | + |
| LoxP | Site misc_feature | 1,264 | 1,297 | 34 | + |
| 35S-Pro | Promoter misc_feature | 1,313 | 1,731 | 419 | + |
| GUS-sense | Other misc_feature | 1,739 | 2,288 | 550 | + |
| Spacer-GUS | Terminator misc_feature | 2,290 | 3,291 | 1,002 | + |
| GUS-AS | Site misc_feature | 0 | 0 | 1 | − |
| NOS-T | Terminator misc_feature | 3,904 | 4,157 | 254 | + |
| LoxP | Site misc_feature | 4,164 | 4,197 | 34 | + |
| BR | Site misc_feature | 4,335 | 4,335 | 1 | + |
| ColE1 | Origin / backbone misc_feature | 4,840 | 5,537 | 698 | + |
| pVS1 ORI | Origin / backbone misc_feature | 6,086 | 9,791 | 3,706 | + |
| Sm/Sp | Selection marker misc_feature | 9,975 | 10,985 | 1,011 | + |
| BL | Site misc_feature | 11,520 | 11,520 | 1 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.