pD1-UbiH-2xStp-RNAi
Ligation of inverted Kpn-UH-Mlu-BsrG* into A251pD1-UbiH-35s-RNAi*
- Promoter 2
- GUBB1HA
- Gene 2
- gus-spacer
- Features
- double promoter construct GUBB1HA with improved termination signals
- Size
- 3,087 bp, circular
- Record date
- 20-JUN-2013
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| UbiH | Promoter misc_feature | 77 | 420 | 344 | + |
| T35S | Terminator misc_feature | 435 | 487 | 53 | − |
| 2xStp | Site misc_signal | 453 | 462 | 10 | + |
| Gus | Coding sequence CDS | 508 | 1,057 | 550 | + |
| unnamed | Coding sequence CDS | 508 | 1,057 | 550 | − |
| T35S | Terminator misc_feature | 1,077 | 1,129 | 53 | + |
| 2xStp | Site misc_signal | 1,102 | 1,111 | 10 | + |
| UbiH | Promoter misc_feature | 1,144 | 1,487 | 344 | − |
| Amp | Selection marker CDS | 1,570 | 2,432 | 863 | + |
| ColE1 | Site misc_feature | 2,433 | 10 | 0 | + |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.