pLN35-Gus-RNAi
Ligation of GusAS into pLN35-GusS-SG
- Promoter 2
- 35S
- Gene 2
- Gus-Spacer-Gus
- Features
- hairpin construct with spacer, Lox sites in cassette
- Size
- 4,761 bp, circular
- Record date
- 13-JUL-2010
Files
Order
Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Send the vector name to info@dna-cloning.com and we ship on invoice.
Annotated features
| Feature | Type | Start | End | Length | Strand |
|---|---|---|---|---|---|
| loxP | Site misc_feature | 46 | 79 | 34 | + |
| P35s | Promoter misc_feature | 95 | 513 | 419 | + |
| GUS-sense | Coding sequence CDS | 521 | 1,070 | 550 | + |
| Spacer-GUS | Terminator misc_feature | 1,072 | 2,073 | 1,002 | + |
| GUS-AS | Site CDS | 0 | 0 | 1 | − |
| Tnos | Terminator misc_feature | 2,686 | 2,939 | 254 | + |
| LoxP | Site misc_feature | 2,946 | 2,979 | 34 | + |
| ColE1 | Site misc_feature | 0 | 0 | 1 | − |
| Amp | Site CDS | 0 | 0 | 1 | − |
PromoterCoding sequenceTerminatorSelection markerOrigin / backboneT-DNA borderSite
Feature types are inferred from the labels in the GenBank file; the file itself is the reference.