RNAi

25 of 31 vectors

Hairpin constructs with intron or spacer for RNA interference in monocots and dicots.

Name	Cassette 1 promoter, gene	Cassette 2 promoter, gene	Features	Files
p35-Gus-iR-BM		35S Gus-iF2-Gus	hairpin construct with intron, Gus RNAi constuct	gbk seq map
p35-iF2-1		35S intron-fad2	hairpin construct with intron, 35S RNAi constuct	gbk seq map
p35-iF2-2		35S intron-fad2	hairpin construct with intron, 35S RNAi constuct	gbk seq map
p6-Ubi-RNAi2	35S hpt	Ubi-maize gus-spacer	double promoter construct for use in monocots	gbk seq map
p6mD-Ubi-RNAi	35s2x hpt-intron-mo	ubi-maize iF2	hairpin construct with intron, for RNAi in monocots, codon optimized hptII for monocots	gbk seq map
p6N-35S-Ubi10-RNAi	Nos hpt	35S gus-spacer	double promoter construct for use in dicots (Arabidopsis+Tobacco)	gbk seq map
p6U10-RNAi	UBQ10AT hpt	35S gus-spacer	double promoter construct for dicots	gbk seq map
p75-GusRNAi	35S bar	35S Gus-spacer-Gus	hairpin construct with spacer, for RNAi	gbk seq map
p7i-Ubi-RNAi2	35S bar	Ubi-maize gus-spacer	double promoter construct for use in monocots	gbk seq map
p7iUA-RNAi	35S bar-intron	Act gus-spacer	double promoter construct for use in monocots	gbk seq map
p7N-35S-Ubi10-RNAi	Nos bar	35S gus-spacer	double promoter construct for use in dicots (Arabidopsis+Tobacco)	gbk seq map
p7P-35Gus-iR	35S bar	35S Gus-intron-Gus	Vector for RNAi of Gus	gbk seq map
p7U-35S-RNAi	Ubi-maize bar	35S gus-spacer	double promoter construct for use in monocots	gbk seq map
p7U10-RNAi	UBQ10AT bar	35S gus-spacer	double promoter construct for dicots	gbk seq map
p9-Nap-RNAi	35S npt	Napin gus-spacer	double promoter construct for use in rapeseed	gbk seq map
p9-Ubi-RNAi2	35S npt	Ubi-maize gus-spacer	double promoter construct for use in monocots	gbk seq map
p9i-UbiH-2xStp-RNAi	35S npt-intron	GUBB1HA gus-spacer	double promoter construct GUBB1HA with improved termination signals	gbk
p9N-35S-Ubi10-RNAi	Nos npt	35S gus-spacer	double promoter construct for use in dicots (Arabidopsis+Tobacco)	gbk seq map
p9U10-RNAi	UBQ10AT npt	35S gus-spacer	double promoter construct for dicots	gbk seq map
pD1-35s-RNAi		35S gus-spacer	double promoter construct 35S	gbk seq map
pD1-35S-RNAi2		35S gus-spacer	double promoter construct 35S	gbk seq map
pD1-35s-Ubi10-RNAi		UBQ10AT gus-spacer	double promoter construct UBQ10AT+35S for dicots	gbk seq map
pD1-Nap-RNAi		35S gus-spacer	double promoter construct 35S	gbk seq map
pLN35-Gus-RNAi		35S Gus-Spacer-Gus	hairpin construct with spacer, Lox sites in cassette	gbk seq map
pLN35-SG		35S Spacer-Gus	hairpin construct with spacer, Lox sites in cassette	gbk seq map

Every vector is sequenced. gbk is the annotated GenBank record, seq the plain sequence, map a drawing of the plasmid. Vectors from the list cost 250 €, Cas9 vectors 400 €, net. Order by e-mail with the vector name.